Simple method for estimating trypsin

Simple method for estimating trypsin.

a simple, fast and commercial method for trypsin purification from bovine pancreas

trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. trypsin has been used widely in various biotechnological processes. in this study trypsin was purified using two precipitation cut, 35% and 75% as saturation percent of ammonium sulfate solutions, tca precipitation (2.5% w/v), ph precipitation and finally affinity chromatography. this m...

A Simple Method For Estimating Conditional Probabilities For SVMs

Support Vector Machines (SVMs) have become a popular learning algorithm, in particular for large, high-dimensional classification problems. SVMs have been shown to give most accurate classification results in a variety of applications. Several methods have been proposed to obtain not only a classification, but also an estimate of the SVMs confidence in the correctness of the predicted label. In...

A Simple Method for Estimating and Testing Minimum-Evolution

A simple method for estimating and testing phylogenetic trees under the principle of minimum evolution (ME) is presented. The basic procedure of this method is first to obtain the neighbor-joining (NJ) tree by Saitou and Nei’s method and then to search for a tree with the minimum value of the sum (S) of branch lengths by examining all trees that are closely related to the NJ tree. Once the ME t...

A simple method for estimating effective ion source residence time.

A method is presented that uses the well-understood O2/Ax ion-molecule reaction system to determine the effective ion source residence time of a chemical ionization source. The process consists of: (1) defining the kinetic system in terms of reactions, reaction rates, and ionization cross sections; (2) solving the differential equations that describe the time evolu-tion of the kinetic system, a...